circLMO7 circRNA bos taurus LMO7 circRNA42 skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of down-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue Overexpression of circLMO7 inhibited the differentiation of primary bovine myoblasts, and it appears to function as a competing endogenous RNA for miR-378a-3p, whose involvement in bovine muscle development has been characterized beforehand. Congruent with our interpretation, circLMO7 increased the number of myoblasts in the S-phase of the cell cycle and decreased the proportion of cells in the G0/G1 phase. Moreover, it promoted the proliferation of myoblasts and protected them from apoptosis. gain of function bovine primary myoblasts miR-378a-3p circRNA-miRNA bovine primary myoblasts luciferase report assay Our analysis of potential miRNA recognition sequences revealed the presence of a putative binding site of miR-378a-3p in circLMO7. In support of this finding, overexpression of circLMO7 significantly decreased the abundance of miR-378a-3p (P<0.05). Moreover, our luciferase based assay found miR-378a-3p to inhibit Rluc expression from psiCHECK2-circLMO7W vectors. HDAC4 circFUT10 circRNA bos taurus FUT10 skeletal muscle development 29044517 CircFUT10 reduces proliferation and facilitates differentiation of myoblasts by sponging miR-133a. Li H, Yang J, Wei X, Song C, Dong D, Huang Y, Lan X, Plath M, Lei C, Ma Y, Qi X, Bai Y, Chen H. J Cell Physiol. 2018 The tissue expression assay showed that the expression of circFUT10 was high in longissimus muscle, and to be much higher (7.43-fold) at the embryonic stage compared with the adult stage, suggesting a potential role in muscle development.We found that circFUT10 was expressed predominantly in muscle and weakly expressed in other tissues. RNA-seq, qRT-PCR longissimus muscle circFUT10 induced bovine primary myoblasts differentiation and increased the expression of MyoD, MyoG, and MyhC in mRNA and protein levels. circFUT10 increased the number of myoblasts in the G0/G1 phase of the cell cycle, and decreased the proportion of cells in the S-phase. circFUT10 inhibited the proliferation of myoblasts and promoted them apoptosis. gain of function bovine primary myoblasts miR-133a circRNA-miRNA bovine primary myoblasts luciferase report assay circFUT10, by binding miR-133a, acts as a decoy to relieve miRNA inhibiting effect on SRF. SRF circRNA262 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of down-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA347 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of down-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA1039 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA1169 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA1170 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA1221 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA124 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA152 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA1945 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA1946 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA1947 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA294 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA297 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA378 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA379 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue circRNA46 circRNA bos taurus skeletal muscle development 29072698 Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p. Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H. Cell Death Dis. 2017 Our qPCR analysis found the presumed patterns of up-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs. RNA-seq, qRT-PCR bovine muscle tissue cCsn1s1(1) circRNA bos taurus Csn1s1 lactation 27040791 Circular RNA of cattle casein genes are highly expressed in bovine mammary gland. Zhang C, Wu H, Wang Y, Zhu S, Liu J, Fang X, Chen H. J Dairy Sci. 2016 expressed at significantly higher levels on lactation d 90 than on lactation d 250. RNA-seq, qRT-PCR the bovine mammary gland cCsn1s1(2) circRNA bos taurus Csn1s1 lactation 27040791 Circular RNA of cattle casein genes are highly expressed in bovine mammary gland. Zhang C, Wu H, Wang Y, Zhu S, Liu J, Fang X, Chen H. J Dairy Sci. 2016 expressed at significantly higher levels on lactation d 90 than on lactation d 250. RNA-seq, qRT-PCR the bovine mammary gland cCsn1s1(3) circRNA bos taurus Csn1s1 lactation 27040791 Circular RNA of cattle casein genes are highly expressed in bovine mammary gland. Zhang C, Wu H, Wang Y, Zhu S, Liu J, Fang X, Chen H. J Dairy Sci. 2016 expressed at significantly higher levels on lactation d 90 than on lactation d 250. RNA-seq, qRT-PCR the bovine mammary gland cCsn1s2 circRNA bos taurus Csn1s2 lactation 27040791 Circular RNA of cattle casein genes are highly expressed in bovine mammary gland. Zhang C, Wu H, Wang Y, Zhu S, Liu J, Fang X, Chen H. J Dairy Sci. 2016 expressed at significantly higher levels on lactation d 90 than on lactation d 250. RNA-seq, qRT-PCR the bovine mammary gland cCsn2 circRNA bos taurus Csn2 lactation 27040791 Circular RNA of cattle casein genes are highly expressed in bovine mammary gland. Zhang C, Wu H, Wang Y, Zhu S, Liu J, Fang X, Chen H. J Dairy Sci. 2016 expressed at significantly higher levels on lactation d 90 than on lactation d 250. RNA-seq, qRT-PCR the bovine mammary gland