cel_circ_0000038 circRNA caenorhabditis elegans afd-1 cel_circ_0000038,cel_circ_008208 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products. Enriched upon RNase R treatment. We set out to confirm two of these high abundance circRNAs using Northern blot analysis: cel_circ_0000038 and cel_circ_0000439. Significantly upregulated in D-10 versus L4. qRT-PCR,sanger sequencing, northern blots the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. cel_circ_0000040 circRNA caenorhabditis elegans abtm-1 cel_circ_0000040,cel_circ_000150 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products. Significantly upregulated in D-10 versus L4. qRT-PCR,sanger sequencing the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. cel_circ_0000075 circRNA caenorhabditis elegans gld-2 cel_circ_0000075,cel_circ_008564 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products. Significantly upregulated in D-10 versus L4. qRT-PCR,sanger sequencing the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. cel_circ_0000105 circRNA caenorhabditis elegans daf-16 cel_circ_0000105,cel_circ_008567 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products. Enriched upon RNase R treatment. qRT-PCR,sanger sequencing the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. circzip-2 circRNA caenorhabditis elegans zip-2 cel_circ_0000308,cel_circ_000006 Parkinson¡¯s disease 29363043 Functional Characterization of Novel Circular RNA Molecule, circzip-2 and Its Synthesizing Gene zip-2 in C. elegans Model of Parkinsons Disease. Kumar L, Shamsuzzama, Jadiya P, Haque R, Shukla S, Nazir A. Mol Neurobiol. 2018 In order to quantify the level of circRNAs circzip-2 and cel_circ_000005 in PD model of C. elegans,we carried out quantitative real-time PCR wherein we observed the downregulation of circzip-2, the expression level of circzip-2 was significantly downregulated by 17.90-fold (p < 0.001) in PD model as compared to wild-type strain of C. elegans. qRT-PCR PD model and wild-type strain of C. elegans miR-60-3p circRNA-miRNA predicted circzip-2 possibly sponges microRNA molecule miR-60 towards asserting an important role in various processes associated with PD. cel_circ_0000308 circRNA caenorhabditis elegans zip-2 cel_circ_0000308,cel_circ_000006 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products. Enriched upon RNase R treatment. Significantly upregulated in D-10 versus L4. qRT-PCR,sanger sequencing the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. cel_circ_0000438 circRNA caenorhabditis elegans crh-1 cel_circ_0000438,cel_circ_000018 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products. Enriched upon RNase R treatment. Significantly upregulated in D-10 versus L4. qRT-PCR,sanger sequencing the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. cel_circ_0000439 circRNA caenorhabditis elegans crh-1 cel_circ_0000439,cel_circ_005708 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products. We set out to confirm two of these high abundance circRNAs using Northern blot analysis: cel_circ_0000038 and cel_circ_0000439. Significantly upregulated in D-10 versus L4. qRT-PCR,sanger sequencing, northern blots the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. cel_circ_0001456 circRNA caenorhabditis elegans daf-2 cel_circ_0001456,cel_circ_008464 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products. Significantly upregulated in D-10 versus L4. qRT-PCR,sanger sequencing the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. cel_circ_new_0005000 circRNA caenorhabditis elegans skn-1 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products qRT-PCR,sanger sequencing the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. cel_circ_new_0005550 circRNA caenorhabditis elegans akt-1 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products qRT-PCR,sanger sequencing the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. cel_circ_new_0005551 circRNA caenorhabditis elegans akt-1 aging 29298683 Global accumulation of circRNAs during aging in Caenorhabditis elegans. Cortés-López M, Gruner MR, Cooper DA, Gruner HN, Voda AI, van der Linden AM, Miura P. BMC Genomics. 2018 The presence of a back-spliced junction was confirmed by Sanger sequencing of RT-PCR products. Significantly upregulated in D-10 versus L4. qRT-PCR,sanger sequencing the C. elegans Bristol N2 wild type strain, L4 larval stage, D-1, D-7 and D-10 of adulthood. cel-circRNA10 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA14 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA16 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA17 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA18 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA19 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA20 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA21 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA3 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA4 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA5 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA6 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA7 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA8 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm cel-circRNA9 circRNA caenorhabditis elegans 23446348 Circular RNAs are a large class of animal RNAs with regulatory potency. Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, Loewer A, Ziebold U, Landthaler M, Kocks C, le Noble F, Rajewsky N. Nature. 2013 The circRNA was validated experimentally. qRT-PCR, sanger sequencing worm