chi_circ_0008219 circRNA capra hircus ovarian follicular development 29107014 Circular RNA profiling reveals chi_circ_0008219 function as microRNA sponges in pre-ovulatory ovarian follicles of goats (Capra hircus). Tao H, Xiong Q, Zhang F, Zhang N, Liu Y, Suo X, Li X, Yang Q, Chen M. Genomics. 2017 Upregulated in the pre-ovulatory follicles of Macheng goat and Boer goat. qRT-PCR, RNA-seq pre-ovulatory follicles of Macheng goat and Boer goat chi-miR-34c-5p,chi-miR-483,chi-miR-1468-3p circRNA-miRNA CHO cells luciferase reporter assay MiRbase software predicted that chi_circ_0008219 contained a putative miRNA binding site. To identify the miRNAs that bind to chi_circ_0008219, a 1788 bp region of chi_circ_0008219 was cloned into the pmirGLO vector. The miRNA mimic of chi-miR-34c-5p, chimiR-483 and chi-miR-1468-3p was co-transfected with the 8219-pmirGLO luciferase reporters into Chinese hamster ovary cell (CHO). Compared with the negative control (NC) miRNA mimic, chi-miR-34c-5p and chi-miR-1468-3p were able to significantly reduce the luciferase reporter activities (P < 0.01) and chi-miR-483 could significantly reduce the luciferase reporter activities (P < 0.05). Three miRNA mutants (chi-miR-34c-5p mut, chi-miR-483 mut and chi-miR-1468-3p mut) were transfected into CHO cells. We discovered that these mutations completely abolished the suppression of luciferase activity compare with the wild-type vector. These results suggest that chi_circ_0008219 may function as a sponge to these miRNAs. chi_circ_0003472 circRNA capra hircus ovarian follicular development 29107014 Circular RNA profiling reveals chi_circ_0008219 function as microRNA sponges in pre-ovulatory ovarian follicles of goats (Capra hircus). Tao H, Xiong Q, Zhang F, Zhang N, Liu Y, Suo X, Li X, Yang Q, Chen M. Genomics. 2017 Downregulated in the pre-ovulatory follicles of Macheng goat and Boer goat. qRT-PCR, RNA-seq pre-ovulatory follicles of Macheng goat and Boer goat chi_circ_0003645 circRNA capra hircus ovarian follicular development 29107014 Circular RNA profiling reveals chi_circ_0008219 function as microRNA sponges in pre-ovulatory ovarian follicles of goats (Capra hircus). Tao H, Xiong Q, Zhang F, Zhang N, Liu Y, Suo X, Li X, Yang Q, Chen M. Genomics. 2017 Downregulated in the pre-ovulatory follicles of Macheng goat and Boer goat. qRT-PCR, RNA-seq pre-ovulatory follicles of Macheng goat and Boer goat chi_circ_0003652 circRNA capra hircus ovarian follicular development 29107014 Circular RNA profiling reveals chi_circ_0008219 function as microRNA sponges in pre-ovulatory ovarian follicles of goats (Capra hircus). Tao H, Xiong Q, Zhang F, Zhang N, Liu Y, Suo X, Li X, Yang Q, Chen M. Genomics. 2017 Downregulated in the pre-ovulatory follicles of Macheng goat and Boer goat. qRT-PCR, RNA-seq pre-ovulatory follicles of Macheng goat and Boer goat chi_circ_0011200 circRNA capra hircus ovarian follicular development 29107014 Circular RNA profiling reveals chi_circ_0008219 function as microRNA sponges in pre-ovulatory ovarian follicles of goats (Capra hircus). Tao H, Xiong Q, Zhang F, Zhang N, Liu Y, Suo X, Li X, Yang Q, Chen M. Genomics. 2017 Downregulated in the pre-ovulatory follicles of Macheng goat and Boer goat. qRT-PCR, RNA-seq pre-ovulatory follicles of Macheng goat and Boer goat chi_circ_0003965 circRNA capra hircus ovarian follicular development 29107014 Circular RNA profiling reveals chi_circ_0008219 function as microRNA sponges in pre-ovulatory ovarian follicles of goats (Capra hircus). Tao H, Xiong Q, Zhang F, Zhang N, Liu Y, Suo X, Li X, Yang Q, Chen M. Genomics. 2017 Upregulated in the pre-ovulatory follicles of Macheng goat and Boer goat. qRT-PCR, RNA-seq pre-ovulatory follicles of Macheng goat and Boer goat chi_circ_0004436 circRNA capra hircus ovarian follicular development 29107014 Circular RNA profiling reveals chi_circ_0008219 function as microRNA sponges in pre-ovulatory ovarian follicles of goats (Capra hircus). Tao H, Xiong Q, Zhang F, Zhang N, Liu Y, Suo X, Li X, Yang Q, Chen M. Genomics. 2017 Upregulated in the pre-ovulatory follicles of Macheng goat and Boer goat. qRT-PCR, RNA-seq pre-ovulatory follicles of Macheng goat and Boer goat chi_circ_0007167 circRNA capra hircus ovarian follicular development 29107014 Circular RNA profiling reveals chi_circ_0008219 function as microRNA sponges in pre-ovulatory ovarian follicles of goats (Capra hircus). Tao H, Xiong Q, Zhang F, Zhang N, Liu Y, Suo X, Li X, Yang Q, Chen M. Genomics. 2017 Upregulated in the pre-ovulatory follicles of Macheng goat and Boer goat. qRT-PCR, RNA-seq pre-ovulatory follicles of Macheng goat and Boer goat circRNA 0000552 circRNA capra hircus muscle 29228601 Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. Oncotarget. 2017 We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ¦Â-Actin was sensitive to the RNase R digestion, and it was completely digested. qRT-PCR,R, RNA-seq sheep muscle circRNA 0000666 circRNA capra hircus muscle 29228601 Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. Oncotarget. 2017 We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ¦Â-Actin was sensitive to the RNase R digestion, and it was completely digested. qRT-PCR, RNA-seq sheep muscle circRNA 0002456 circRNA capra hircus muscle 29228601 Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. Oncotarget. 2017 We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ¦Â-Actin was sensitive to the RNase R digestion, and it was completely digested. qRT-PCR, RNA-seq sheep muscle circRNA 0003541 circRNA capra hircus muscle 29228601 Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. Oncotarget. 2017 We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ¦Â-Actin was sensitive to the RNase R digestion, and it was completely digested. qRT-PCR, RNA-seq sheep muscle circRNA 0004676 circRNA capra hircus muscle 29228601 Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. Oncotarget. 2017 We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ¦Â-Actin was sensitive to the RNase R digestion, and it was completely digested. qRT-PCR, RNA-seq sheep muscle circRNA 0004690 circRNA capra hircus muscle 29228601 Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. Oncotarget. 2017 We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ¦Â-Actin was sensitive to the RNase R digestion, and it was completely digested. qRT-PCR, RNA-seq sheep muscle circRNA 0005179 circRNA capra hircus muscle 29228601 Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. Oncotarget. 2017 We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ¦Â-Actin was sensitive to the RNase R digestion, and it was completely digested. qRT-PCR, RNA-seq sheep muscle circRNA 0005243 circRNA capra hircus muscle 29228601 Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. Oncotarget. 2017 We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ¦Â-Actin was sensitive to the RNase R digestion, and it was completely digested. qRT-PCR, RNA-seq sheep muscle circRNA 0005250 circRNA capra hircus muscle 29228601 Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. Oncotarget. 2017 We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ¦Â-Actin was sensitive to the RNase R digestion, and it was completely digested. qRT-PCR, RNA-seq sheep muscle circRNA 0005256 circRNA capra hircus muscle 29228601 Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. Oncotarget. 2017 We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ¦Â-Actin was sensitive to the RNase R digestion, and it was completely digested. qRT-PCR, RNA-seq sheep muscle circ-0000198 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0000428 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0000469 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0001076 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0001216 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0001282 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0001655 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0001993 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0001995 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0002794 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0003004 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0003088 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0003092 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0003094 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0003479 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0004352 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0004460 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0004489 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0004723 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0005181 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0005695 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0005738 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0006581 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0007204 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0007336 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0007685 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0007685 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0008710 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland circ-0009865 circRNA capra hircus growth and development of the pituitary gland 29170496 Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. Sci Rep. 2017 To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ¦Â-actin mRNA was detected (sensitive to RNase R). qRT-PCR, RNA-seq sheep pituitary gland