RefCirc
a reference database for circRNAs validated by experiments

circ-Foxo3 Details
Basic Information
circRNA    circ-Foxo3
Alias    -
circBase ID    -
Host gene    Foxo3
Species    mus musculus
Peptide    -
Sequence  -
Expression
Description  highly expressed in non-cancer cells
Method    qRT-PCR, northern blots
Sample    MEF, MCF, NIH3T3, 67NR, 66C14, 4T07, 4T1 and B16
Function
Description  Silencing endogenous circ-Foxo3 promoted cell proliferation. Ectopic expression of circ-Foxo3 repressed cell cycle progression by binding to the cell cycle proteins cyclin-dependent kinase 2 (also known as cell division protein kinase 2 or CDK2) and cyclin-dependent kinase inhibitor 1 (or p21), resulting in the formation of a ternary complex.
Method    loss of function, gain of function
in vitro    NIH3T3, B16
in vivo    -
Interaction
Target    p21 and CDK2
Type    circRNA-protein
Sample    NIH3T3
Experiment    RIP, RNA pull-down
Description    The experiment showed that circ-Foxo3 was pulled-down by immunoprecipitation experiments with antibodies against CDK2 and p21, which did not pull-down linear Foxo3 mRNA. p21 and CDK2 were pulleddown by the probe but not by the control oligo in the circ-Foxo3-transfected cells.
ceRNA target    -
Reference
Pubmed ID    26861625
Trait/Disease    -
Title    Foxo3 circular RNA retards cell cycle progression via forming ternary complexes with p21 and CDK2.
Authors    Du WW, Yang W, Liu E, Yang Z, Dhaliwal P, Yang BB.
Journal    Nucleic Acids Res. 2016