RefCirc
a reference database for circRNAs validated by experiments

circ-Foxo3 Details
Basic Information
circRNA    circ-Foxo3
Alias    -
circBase ID    -
Host gene    Foxo3
Species    mus musculus
Peptide    -
Sequence  -
Expression
Description  highly expressed in heart samples of aged patients and mice
Method    northern blots, RT-PCR, qRT-PCR, FISH
Sample    MEF, primary cardiomyocytes
Function
Description  ID-1, E2F1, FAK, and HIF1?? interact with circ-Foxo3 and are retained in the cytoplasm and could no longer exert their anti-senescent and anti-stress roles, resulting in increased cellular senescence.
Method    loss of function, gain of function
in vitro    MEF, primary cardiomyocytes
in vivo    6-8 weeks, BALB/c strain
Interaction
Target    ID1, E2F1, HIF1a, and FAK
Type    circRNA-protein
Sample    MEF, primary cardiomyocytes
Experiment    RIP, RNA pull-down
Description    circ-Foxo3 was pulled down by antibodies against ID1, E2F1, FAK, and HIF1a, but the linear Foxo3 mRNA was not. It was found that circ-Foxo3 could pull down Id1, E2F1, HIF1a, and FAK from cells transfected with circ-Foxo3.
ceRNA target    -
Reference
Pubmed ID    26873092
Trait/Disease    -
Title    Foxo3 circular RNA promotes cardiac senescence by modulating multiple factors associated with stress and senescence responses.
Authors    Du WW, Yang W, Chen Y, Wu ZK, Foster FS, Yang Z, Li X, Yang BB.
Journal    Eur Heart J. 2017