RefCirc
a reference database for circRNAs validated by experiments

Cdr1as Details
Basic Information
circRNA    Cdr1as
Alias    -
circBase ID    -
Host gene    Cdr1
Species    mus musculus
Peptide    -
Sequence  -
Expression
Description  Cdr1as was highly expressed in neurons but not expressed in glial cells such as oligodendrocytes and astrocytes. Cdr1as was predominantly expressed in excitatory neurons. In the cerebellum, Cdr1as expression was observed exclusively in the granular layer with a high content of excitatory neurons and did not overlap with GABAergic neurons present in the molecular layer and Purkinje cells. Single molecule RNA FISH in primary cortical neurons revealed Cdr1as expression in both soma and neurites.
Method    FISH
Sample    neurons, glial cells such as oligodendrocytes and astrocytes, excitatory neurons, cerebellum, soma and neurites.
Function
Description  When the Cdr1as locus was removed from the mouse genome, knockout animals displayed impaired sensorimotor gating a deficit in the ability to filter out unnecessary information which is associated with neuropsychiatric disorders. Electrophysiological recordings revealed dysfunctional synaptic transmission. Expression of miR-7 and miR-671 was specifically and posttranscriptionally misregulated in all brain regions analyzed. Expression of immediate early genes such as Fos, a direct miR-7 target, was enhanced in Cdr1as-deficient brains, providing a possible molecular link to the behavioral phenotype.
Method    loss of function
in vitro    -
in vivo    Cdr1as loss-of-function mutant mice
Interaction
Target    miR-7 and miR-671
Type    circRNA-miRNA
Sample    cerebellum, cortex, hippocampus, and olfactory bulb
Experiment    Northern blots, FISH, and chromogenic ISH
Description    there is a highly specific, posttranscriptional deregulation of miR-7 and miR-671 in the brains of Cdr1as KO animals. These are the two miRNAs that we identified by in vivo chimera analysis as directly interacting with Cdr1as.
ceRNA target    Fos
Reference
Pubmed ID    28798046
Trait/Disease    Neuropsychiatric Disorder
Title    Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function.
Authors    Piwecka M, Gla??ar P, Hernandez-Miranda LR, Memczak S, Wolf SA, Rybak-Wolf A, Filipchyk A, Klironomos F, Cerda Jara CA, Fenske P, Trimbuch T, Zywitza V, Plass M, Schreyer L, Ayoub S, Kocks C, K??hn R, Rosenmund C, Birchmeier C, Rajewsky N.
Journal    Science. 2017