RefCirc
a reference database for circRNAs validated by experiments

circ-Ttn Details
Basic Information
circRNA    circ-Ttn
Alias    -
circBase ID    -
Host gene    Ttn
Species    mus musculus
Peptide    -
Sequence  -
Expression
Description  Significantly changed in pLV (plasmid Lentivirus) empty, pLV Qki5, CRi empty, CRi 3+4 Qki, and CRi 5+6 Qki HL-1 cell lines. Positively regulated with Qki expression. Downregulated with doxorubicin treatment in HL-1 cells. Downregulated in mouse hearts, which had doxorubicin-induced reductions in Qki levels. Downregulated in control (pLV empty) cells exposed to doxorubicin compared with pLV Qki5-overexpressing cells.
Method    qRT-PCR
Sample    HL-1 cell lines
Function
Description  we inhibited Ttn 105-111 circular RNA by a specific siRNA approach (Figure 3G) and evaluated its effect on doxorubicin-induced apoptosis. SiRNA mediated inhibition of Ttn 105-111 led to increased susceptibility to doxorubicin as evident by higher caspase activity. Additionally, the knockdown of Ttn 105-111 in pLV Qki5-overexpressing cell line also resulted in an induced caspase activity. On the contrary, lentiviral mediated overexpression of circular RNA Ttn 105-111 resulted in lower caspase 3/7 activity and increased survival as seen by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (Online Figure IVD though IVF).
Method    loss of function, gain of function
in vitro    HL-1 cell lines
in vivo    -
Interaction
Target    -
Type    -
Sample    -
Experiment    -
Description    -
ceRNA target    -
Reference
Pubmed ID    29133306
Trait/Disease    Doxorubicin-Mediated Cardiotoxicity
Title    Quaking Inhibits Doxorubicin-Mediated Cardiotoxicity Through Regulation of Cardiac Circular RNA Expression.
Authors    Gupta SK, Garg A, Bar C, Chatterjee S, Foinquinos A, Milting H, Streckfub-Bomeke K, Fiedler J, Thum T.
Journal    Circ Res. 2018