RefCirc
a reference database for circRNAs validated by experiments

circHECTD1 Details
Basic Information
circRNA    circHECTD1
Alias    mm9_circ_002888
circBase ID    mmu_circ_0000375
Host gene    Hectd1
Species    mus musculus
Peptide    -
Sequence  GAAGAAGAAGAGTATGAAACCAAAGGAGGCCGCCGCAGAGCCTGGGATGACGACTATGTGCTAAAGCGCCAGTTTTCTGCACTGGTCCCTGCTTTTGATCCTAGACCTGGTCGTACCAATGTCCAGCAGACAACTGACCTAGAAATTCCTCCCCCAGGAACACCTCACTCAGAGCTCTTGGAGGAAGTTGAATGTACTCCGTCACCTCGCTTGGCTCTCACACTGAAAGTGACGGGGCTTGGAACAACGCGGGAAGTTGAACTGCCACTTACCAATTTCAGATCCACCATCTTTTACTATGTACAAAAACTGCTTCAACTGTCTTGTAATGGCAATGTGAAGTCAGATAAACTTAGGCGTATTTGGGAGCCCACTTACAC
Expression
Description  SiO2 induced a rapid increase in circHECTD1 expression in MML1 cells, which was confirmed using FISH. Moreover, SiO2 also induced circHECTD1 expression in human endothelial cell line-HUVECs.
Method    qRT-PCR, FISH
Sample    MML1 cells, human endothelial cell line-HUVECs
Function
Description  SiO2 concomitantly increased circHECTD1 expression, which, in turn, inhibited HECTD1 protein expression. SiO2-induced increases in cell proliferation, migration, and changes in marker levels were restored by either a small interfering RNA (siRNA) targeting circHECTD1 or overexpression of HECTD1 via the CRISPR/Cas9 system, confirming the involvement of the circHECTD1/HECTD1 pathway in the EndMT.
Method    loss of function
in vitro    MML1
in vivo    Tie2-GFP mice exposed to SiO2 and silicosis patients
Interaction
Target    -
Type    -
Sample    -
Experiment    -
Description    -
ceRNA target    -
Reference
Pubmed ID    29540674
Trait/Disease    Pulmonary Silicosis
Title    circHECTD1 promotes the silica-induced pulmonary endothelial-mesenchymal transition via HECTD1.
Authors    Fang S, Guo H, Cheng Y, Zhou Z, Zhang W, Han B, Luo W, Wang J, Xie W, Chao J.
Journal    Cell Death Dis. 2018