RefCirc
a reference database for circRNAs validated by experiments

chi_circ_0008219 Details
Basic Information
circRNA    chi_circ_0008219
Alias    -
circBase ID    -
Host gene    -
Species    capra hircus
Peptide    -
Sequence  -
Expression
Description  Upregulated in the pre-ovulatory follicles of Macheng goat and Boer goat.
Method    qRT-PCR, RNA-seq
Sample    pre-ovulatory follicles of Macheng goat and Boer goat
Function
Description  -
Method    -
in vitro    -
in vivo    -
Interaction
Target    chi-miR-34c-5p,chi-miR-483,chi-miR-1468-3p
Type    circRNA-miRNA
Sample    CHO cells
Experiment    luciferase reporter assay
Description    MiRbase software predicted that chi_circ_0008219 contained a putative miRNA binding site. To identify the miRNAs that bind to chi_circ_0008219, a 1788 bp region of chi_circ_0008219 was cloned into the pmirGLO vector. The miRNA mimic of chi-miR-34c-5p, chimiR-483 and chi-miR-1468-3p was co-transfected with the 8219-pmirGLO luciferase reporters into Chinese hamster ovary cell (CHO). Compared with the negative control (NC) miRNA mimic, chi-miR-34c-5p and chi-miR-1468-3p were able to significantly reduce the luciferase reporter activities (P < 0.01) and chi-miR-483 could significantly reduce the luciferase reporter activities (P < 0.05). Three miRNA mutants (chi-miR-34c-5p mut, chi-miR-483 mut and chi-miR-1468-3p mut) were transfected into CHO cells. We discovered that these mutations completely abolished the suppression of luciferase activity compare with the wild-type vector. These results suggest that chi_circ_0008219 may function as a sponge to these miRNAs.
ceRNA target    -
Reference
Pubmed ID    29107014
Trait/Disease    Ovarian Follicular Development
Title    Circular RNA profiling reveals chi_circ_0008219 function as microRNA sponges in pre-ovulatory ovarian follicles of goats (Capra hircus).
Authors    Tao H, Xiong Q, Zhang F, Zhang N, Liu Y, Suo X, Li X, Yang Q, Chen M.
Journal    Genomics. 2017