RefCirc
a reference database for circRNAs validated by experiments
| circ-0001282 Details |
| Basic Information |
| circRNA | circ-0001282 |
| Alias | - |
| circBase ID | - |
| Host gene | - |
| Species | capra hircus |
| Peptide | - |
| Sequence | - |
| Expression |
| Description | To confirm the data on circRNAs generated from RNA-seq analysis, we further experimentally detected the expression of sheep circRNAs. To validate our sequencing data, divergent primers were designed to amplify the circular junctions. Out of 30 randomly selected circRNAs, 29 junctions (except for circ-9563) showed the expected band sizes by RT-PCR analysis. Some reactions generated nonspecific products, which could be isoforms of alternative splicing circularization. Head-to-tail junctions were confirmed by DNA sequencing. We also tested the resistance of circRNAs to RNase R digestion by real-time RT-PCR. All five tested circRNAs showed resistance to RNase R digestion, whereas no linear ??-actin mRNA was detected (sensitive to RNase R). |
| Method | qRT-PCR, RNA-seq |
| Sample | sheep pituitary gland |
| Reference |
| Pubmed ID | 29170496 |
| Trait/Disease | Pituitary Gland Growth and Development |
| Title | Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep. |
| Authors | Li C, Li X, Ma Q, Zhang X, Cao Y, Yao Y, You S, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Ni W, Hu S. |
| Journal | Sci Rep. 2017 |