RefCirc
a reference database for circRNAs validated by experiments
| circRNA 0005256 Details |
| Basic Information |
| circRNA | circRNA 0005256 |
| Alias | - |
| circBase ID | - |
| Host gene | - |
| Species | capra hircus |
| Peptide | - |
| Sequence | - |
| Expression |
| Description | We randomly selected 10 circRNAs and designed divergent primers across the circRNAs junctions. RT-PCR amplification and DNA sequencing techniques were used to confirm the circRNAs data of RNA-seq. The results of the RT-PCR amplification show a single band of the expected size. DNA sequencing results confirm the head-totail circularization splicing of these circRNAs. Real-Time RT-PCR was further used for verifying circRNAs resistance to RNase R digestion experiments. All selected 10 circRNAs showed resistance to RNase R digestion. As expected, linear control of ??-Actin was sensitive to the RNase R digestion, and it was completely digested. |
| Method | qRT-PCR, RNA-seq |
| Sample | sheep muscle |
| Reference |
| Pubmed ID | 29228601 |
| Trait/Disease | Muscle |
| Title | Genome-wide analysis of circular RNAs in prenatal and postnatal muscle of sheep. |
| Authors | Li C, Li X, Yao Y, Ma Q, Ni W, Zhang X, Cao Y, Hazi W, Wang D, Quan R, Hou X, Liu Z, Zhan Q, Liu L, Zhang M, Yu S, Hu S. |
| Journal | Oncotarget. 2017 |