RefCirc
a reference database for circRNAs validated by experiments
| bmo_circ_0002460 Details |
| Basic Information |
| circRNA | bmo_circ_0002460 |
| Alias | bmo_circ_0002460 |
| circBase ID | - |
| Host gene | - |
| Species | bombyx mori |
| Peptide | - |
| Sequence | - |
| Expression |
| Description | We further experimentally validated the candidate B. mori circRNAs. Convergent and divergent primers were designed to amplify each circRNA in the RNase R-treated sample (cDNA) and genomic DNA. Convergent primers were designed as a positive control for the corresponding linear transcripts, and divergent primers were designed to detect the circular template. Theoretically, convergent primers should amplify products from both the cDNA and genomic DNA, whereas the divergent primers would only amplify circRNA from cDNA and would fail to amplify a product from the genomic DNA. The reverse transcription PCR amplification results showed that 16 of 38 randomly chosen circRNA were validated successfully. |
| Method | RT-PCR, RNA-seq |
| Sample | middle silk gland (MSG) and posterior silk gland (PSG) |
| Reference |
| Pubmed ID | 28918159 |
| Trait/Disease | Silk Gland |
| Title | Identification of circular RNA in the Bombyx mori silk gland. |
| Authors | Gan H, Feng T, Wu Y, Liu C, Xia Q, Cheng T. |
| Journal | Insect Biochem Mol Biol. 2017 |