RefCirc
a reference database for circRNAs validated by experiments

circLMO7 Details
Basic Information
circRNA    circLMO7
Alias    circRNA42
circBase ID    -
Host gene    LMO7
Species    bos taurus
Peptide    -
Sequence  -
Expression
Description  Our qPCR analysis found the presumed patterns of down-regulation o6f the circRNA to be remarably similar in both types of analysis, suggesting that circRNA-Seq data provided reliable information about the relative abundance of circRNAs.
Method    RNA-seq, qRT-PCR
Sample    bovine muscle tissue
Function
Description  Overexpression of circLMO7 inhibited the differentiation of primary bovine myoblasts, and it appears to function as a competing endogenous RNA for miR-378a-3p, whose involvement in bovine muscle development has been characterized beforehand. Congruent with our interpretation, circLMO7 increased the number of myoblasts in the S-phase of the cell cycle and decreased the proportion of cells in the G0/G1 phase. Moreover, it promoted the proliferation of myoblasts and protected them from apoptosis.
Method    gain of function
in vitro    bovine primary myoblasts
in vivo    -
Interaction
Target    miR-378a-3p
Type    circRNA-miRNA
Sample    bovine primary myoblasts
Experiment    luciferase report assay
Description    Our analysis of potential miRNA recognition sequences revealed the presence of a putative binding site of miR-378a-3p in circLMO7. In support of this finding, overexpression of circLMO7 significantly decreased the abundance of miR-378a-3p (P<0.05). Moreover, our luciferase based assay found miR-378a-3p to inhibit Rluc expression from psiCHECK2-circLMO7W vectors.
ceRNA target    HDAC4
Reference
Pubmed ID    29072698
Trait/Disease    Skeletal Muscle Development
Title    Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p.
Authors    Wei X, Li H, Yang J, Hao D, Dong D, Huang Y, Lan X, Plath M, Lei C, Lin F, Bai Y, Chen H.
Journal    Cell Death Dis. 2017