RefCirc
a reference database for circRNAs validated by experiments

ciRS-7 Details
Basic Information
circRNA    ciRS-7
Alias    CDR1as
circBase ID    hsa_circ_0001946
Host gene    CDR1
Species    homo sapiens
Peptide    -
Sequence  GGTTTCCGATGGCACCTGTGTCAAGGTCTTCCAACAACTCCGGGTCTTCCAGCGACTTCAAGTCTTCCAATAATCTCAAGGTCTTCCAGATAATCCTGAGCTTCCAGAAAATCCACATCTTCCAGACAATCCATGTCTTCCGGACAATCCATGTCTTCCAAGAAGCTCCAAGTCTTCCAGTAAATCAAGTCTTCCAGCAAATCCAGTCTTCCAGCAATTACTGGTCTTCCACCAAATCCAGATCTTCCAGGAAAATCCACGTCTTCCAGGAAATCCATGTCTTCCAATAATTTCAAGGTCTTCCATCAAATACAGATCTTCCAGCTAATCCATGTCTTCCAGAAAAATCTGTGTCTTCCACCAAATCCAAGTCTTCCAGTAAATCTAGTTCTTCCAGAAAAATCTAGATCTTCCAGTCAATCAGTGTCTTCCAGAAAGAAATCCAGGTCTTCCAGTCAATCAGTGTCTTCCAGAAAGAAATCCAGGTCTTCCAGTCAGTCAGTGTCTTCCAGAAAAATCTACGTCTTCCACCAAATCCAGGTCTTCCAGTCAATCCACATCTTCCGGAAAAAATCCAGGTCTTCCAGCCAATATATGTCTTCCTGAAGATCCACGTCTTCCAGAAAATCCATGTCTTCCAGAAAATCCATGTCTTCCAGTAACCTCCCAGTCTTCCAGAAAATCCACGTCTTCCCAACAATCCAAGTCTTCCGGATAATTTGGGTCTTCCTGAAAATCTACGTCTTCCAAAAAAGCCATGTCTTCCAGAAAATCCACATCTTCCAATGGCCTCCAGGTCTTCCAGACTATCCATGTCTTCCAGAAAATCCTTGTCTTCCCTTAAATCTATAGCTTCCAAAAAATCCGGGTCTTCCAGGAAATCCGTGTCTTCCAGCAAGTCCACGTCTTCCAACAAAGCCATGTCTTCCAGACTATCCATGTCTTCCAGAAAATCCTTGTCTTCCCTCAAATCCATAGCTTCCGAAAAATCCAGGTCTTCCAGGAAATCCGTGTCTTCCAGCAAATCCACGTCTTCCAACAAAGCCATGTCTTCCATCAAATTAATGTCTTCCAGCCTACTTGTGTCTTCCAACAAAGGTACGTCTTCCAACAAAGGTACGTCTTCCAACAAAGGTATGTCTTCCAACAAAGGTACGTCTTCCAGAAAATCCACGTCTTCCAACCAAGCCATGTCTTCCAGAAAATCCACGTCTTCCAGAAAATATATGTCTTCCAACTAAGCTACGTCTTCCAACAAATCCATGTCTTCCTATATCTCCAGGTCTTCCAGCATCTCCAGGGCTTCCAGCATCTGCTCGTCTTCCAACATCTCCACGTCTTCCAGCATCTCTGTGTCTTCCAGCATCTTCATGTCTTCCAACAACTACCCAGTCTTCCATCAACTGGCTCAATATCCATGTCTTCCAACGTCTCCAGTGTGCTGATCTTCTGACATTCAGGTCTTCCAGTGTCTGCAATATCCAG
Expression
Description  As in HEK293 cells, the stably expressed ciRS-7 in HeLa cells was sensitive towards miR-671 as shown previously, but remained resistant to miR-7.
Method    qRT-PCR, northern blots
Sample    HEK293, HeLa, mouse brain tissue
Function
Description  Although the circRNA (ciRS-7) is completely resistant to miRNA-mediated target destabilization, it strongly suppresses miR-7 activity, resulting in increased levels of miR-7 targets.
Method    -
in vitro    HEK293, HeLa
in vivo    C57BL/6 male mice
Interaction
Target    miR-7
Type    circRNA-miRNA
Sample    HEK293, HeLa
Experiment    northern blots, qRT-PCR
Description    ciRS-7 contains more than 70 selectively conserved miRNA target sites, and it is highly and widely associated with Argonaute (AGO) proteins in a miR-7-dependent manner.
ceRNA target    SNCA, EGFR, IRS2
Reference
Pubmed ID    23446346
Trait/Disease    -
Title    Natural RNA circles function as efficient microRNA sponges.
Authors    Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK, Kjems J.
Journal    Nature. 2013