RefCirc
a reference database for circRNAs validated by experiments

cir-ITCH Details
Basic Information
circRNA    cir-ITCH
Alias    -
circBase ID    -
Host gene    ITCH
Species    homo sapiens
Peptide    -
Sequence  -
Expression
Description  significantly decreased in lung cancer
Method    RT-PCR, qRT-PCR
Sample    lung cancer biopsy specimens and paired adjacent normal tissues, A549 and NCI-H460
Function
Description  Ectopic expression of cir-ITCH markedly elevated its parental cancer-suppressive gene, ITCH, expression and inhibited proliferation of lung cancer cells. Molecular analysis further revealed that cir-ITCH acted as sponge of oncogenic miR-7 and miR-214 to enhance ITCH expression and thus suppressed the activation of Wnt/??-cateninsignaling.
Method    gain of function
in vitro    A549 and NCI-H460
in vivo    -
Interaction
Target    miR-7 and miR-214
Type    circRNA-miRNA
Sample    A549 and NCI-H460
Experiment    luciferase reporter assay
Description    In both A549 cells andNCI-H460 cells transfected with empty vector (control of cir-ITCH plasmid), luciferase activity was significantly decreased in a concentration-dependent manner with the presence of miR-7/miR-214 mimic. However, there were no significant ifferences in luciferase activity of miR-7/miR-214 mimic in ells with cir-ITCH hyperexpression.
ceRNA target    ITCH
Reference
Pubmed ID    27642589
Trait/Disease    Lung Cancer
Title    Circular RNA-ITCH Suppresses Lung Cancer Proliferation via Inhibiting the Wnt/??-Catenin Pathway.
Authors    Wan L, Zhang L, Fan K, Cheng ZX, Sun QC, Wang JJ.
Journal    Biomed Res Int. 2016