RefCirc
a reference database for circRNAs validated by experiments

circ-Foxo3 Details
Basic Information
circRNA    circ-Foxo3
Alias    -
circBase ID    -
Host gene    Foxo3
Species    homo sapiens
Peptide    -
Sequence  -
Expression
Description  In patient tumor samples and in a panel of cancer cells, circ-Foxo3 was minimally expressed. During cancer cell apoptosis, the expression of circ-Foxo3 was found to be significantly increased.
Method    qRT-PCR
Sample    specimens of patients with breast carcinoma and benign tissues, 293T, Beas2B, HaCaT, NIH3T3, MEF, MCF-10A, MB-468, MB-231, 67NR, 66C14, 4T07, 4T1, B16
Function
Description  Silencing endogenous circ-Foxo3 enhanced cell viability, whereas ectopic expression of circ-Foxo3 triggered stress-induced apoptosis and inhibited the growth of tumor xenografts.
Method    loss of function, gain of function
in vitro    66C14, 4T1, MDA-MB-468, and MDA-MB-231
in vivo    regular Balb/c mice, nude mice, four-week-old C57 mice
Interaction
Target    MDM2 and p53
Type    circRNA-protein
Sample    MB-231
Experiment    FISH, NPDock, pull-down
Description    In situ hybridization displayed colocalization of circ-Foxo3 with Mdm2, p53, Foxo3, and Puma. The molecular simulation result supported that circ-Foxo3 could perfectly dock MDM2 and predicted a minimal binding region of circ-Foxo3 for the MDM2 as agucgauucaugcggguccagaaugagggaacuggcaaga. The molecular simulation result supported that circ-Foxo3 could perfectly dock p53 and predicts a minimal binding region of circ-Foxo3 for p53 as gggugccaggcugaaggaucacug. We found that the biotinylated probe pulled down significantly lower levels of circ-Foxo3 when the cells were transfected with the siRNA.
ceRNA target    -
Reference
Pubmed ID    27886165
Trait/Disease    Apoptosis
Title    Induction of tumor apoptosis through a circular RNA enhancing Foxo3 activity.
Authors    Du WW, Fang L, Yang W, Wu N, Awan FM, Yang Z, Yang BB.
Journal    Cell Death Differ. 2017