RefCirc
a reference database for circRNAs validated by experiments

circR-284 Details
Basic Information
circRNA    circR-284
Alias    hsa_circ_000016
circBase ID    hsa_circ_0000284
Host gene    HIPK3
Species    homo sapiens
Peptide    -
Sequence  GTATGGCCTCACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGAAGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAGGAAAGAAACTATCCACGGACCTATGTGAATGGTAGAAACTTTGGAAATTCTCATCCTCCCACTAAGGGTAGTGCTTTTCAGACAAAGATACCATTTAATAGACCTCGAGGACACAACTTTTCATTGCAGACAAGTGCTGTTGTTTTGAAAAACACTGCAGGTGCTACAAAGGTCATAGCAGCTCAGGCACAGCAAGCTCACGTGCAGGCACCTCAGATTGGGGCGTGGCGAAACAGATTGCATTTCCTAGAAGGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAATCATAGCAGCGCAATGCAGATTGTCGATGAATTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAATCCAGTGACAGTTGTGACAGCTACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGTTAGTACAGCATGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTTGATTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAGAGGGACAAATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTTATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGCTGATGAATATAACTTTGTACGAGCTTATGAATGCTTTCAGCACCGTAACCATACTTGTTTAGTCTTTGAGATGCTGGAACAAAACTTGTATGACTTTCTGAAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGGCCCATTCTTCAACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGGTTTAATTCATGCTGATCTCAAGCCAGAGAATATTATGTTGGTGGATCCTGTTCGGCAGCCTTACAGGGTTAAAGTAATAGACTTTGGGTCGGCCAGTCATGTATCAAAGACTGTTTGTTCAACATATCTACAATCTCGGTACTACAG
Expression
Description  We confirmed that circR-284 is expressed in human VSMCs and in carotid plaques. Serum circR-284 levels did not exhibit a significant change after an acute carotid plaque rupture-mediated ischemic cerebrovascular event, such as transient ischemic attack or stroke (2.96+/-1.16 versus 1.00+/-0.37; P=0.06). The ratio of serum circR-284:miR-221 was significantly elevated in the urgent group (P=0.0002) and exhibited favorable characteristics as a biomarker indicative of carotid plaque rupture and stroke. A validation study in 112 patients (47 asymptomatic, 41 urgent, and 24 patients with a cerebrovascular event between 5 and 180 days of the carotid endarterectomy [symptomatic]) confirmed elevation of serum circR-284:miR-221.
Method    qRT-PCR
Sample    human VSMCs, carotid plaques
Function
Description  -
Method    -
in vitro    -
in vivo    -
Interaction
Target    miR-221 and miR-222
Type    circRNA-miRNA
Sample    -
Experiment    predicted
Description    circR-284 (circbase.org) possesses an miR-221 and miR-222 binding site and may serve to regulate miR-221/miR-222 activity.
ceRNA target    -
Reference
Pubmed ID    28779016
Trait/Disease    Carotid Plaque Rupture
Title    Carotid Plaque Rupture Is Accompanied by an Increase in the Ratio of Serum circR-284 to miR-221 Levels.
Authors    Bazan HA, Hatfield SA, Brug A, Brooks AJ, Lightell DJ Jr, Woods TC.
Journal    Circ Cardiovasc Genet. 2017