RefCirc
a reference database for circRNAs validated by experiments

CircHIPK3 Details
Basic Information
circRNA    CircHIPK3
Alias    BCRC-2,hsa_circ_000016
circBase ID    hsa_circ_0000284
Host gene    HIPK3
Species    homo sapiens
Peptide    -
Sequence  GTATGGCCTCACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGAAGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAGGAAAGAAACTATCCACGGACCTATGTGAATGGTAGAAACTTTGGAAATTCTCATCCTCCCACTAAGGGTAGTGCTTTTCAGACAAAGATACCATTTAATAGACCTCGAGGACACAACTTTTCATTGCAGACAAGTGCTGTTGTTTTGAAAAACACTGCAGGTGCTACAAAGGTCATAGCAGCTCAGGCACAGCAAGCTCACGTGCAGGCACCTCAGATTGGGGCGTGGCGAAACAGATTGCATTTCCTAGAAGGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAATCATAGCAGCGCAATGCAGATTGTCGATGAATTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAATCCAGTGACAGTTGTGACAGCTACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGTTAGTACAGCATGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTTGATTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAGAGGGACAAATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTTATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGCTGATGAATATAACTTTGTACGAGCTTATGAATGCTTTCAGCACCGTAACCATACTTGTTTAGTCTTTGAGATGCTGGAACAAAACTTGTATGACTTTCTGAAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGGCCCATTCTTCAACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGGTTTAATTCATGCTGATCTCAAGCCAGAGAATATTATGTTGGTGGATCCTGTTCGGCAGCCTTACAGGGTTAAAGTAATAGACTTTGGGTCGGCCAGTCATGTATCAAAGACTGTTTGTTCAACATATCTACAATCTCGGTACTACAG
Expression
Description  The expression of circHIPK3 was obviously decreased in 79.5% of the bladder cancer tissues compared with normal bladder tissues and was negatively correlated with bladder cancer grade, invasion, and the lymph nodes metastasis, respectively. Downregulation of circHIPK3 was also found in human muscle-invasive bladder cancer cells T24T and UMUC3 compared with human immortalized uroepithelium cells SV-HUC-1. circHIPK3 is relatively low-expressed in bladder cancer tissues and cell lines, and predominantly localized in cytoplasm.
Method    RNA-seq, qRT-PCR, northern blots, FISH
Sample    bladder cancer tissues, normal bladder tissues, T24T, UMUC3, SV-HUC-1
Function
Description  Over-expression of circHIPK3 effectively inhibits migration, invasion, and angiogenesis of bladder cancer cells in vitro and suppresses bladder cancer growth and metastasis in vivo. Mechanistic studies reveal that circHIPK3 contains two critical binding sites for the microRNA miR-558 and can abundantly sponge miR-558 to suppress the expression of heparanase (HPSE).
Method    loss of function, gain of function
in vitro    T24T, UMUC3
in vivo    female BALB/c nude mice
Interaction
Target    miR-558
Type    circRNA-miRNA
Sample    T24T and UMUC3 cells
Experiment    RNA pull-down, FISH
Description    The above results demonstrate that circHIPK3 can directly bind to miR-558 in T24T and UMUC3 cells.
ceRNA target    HPSE
Reference
Pubmed ID    28794202
Trait/Disease    Bladder Cancer
Title    CircHIPK3 sponges miR-558 to suppress heparanase expression in bladder cancer cells.
Authors    Li Y, Zheng F, Xiao X, Xie F, Tao D, Huang C, Liu D, Wang M, Wang L, Zeng F, Jiang G.
Journal    EMBO Rep. 2017