RefCirc
a reference database for circRNAs validated by experiments

KIRKOS-71 Details
Basic Information
circRNA    KIRKOS-71
Alias    -
circBase ID    hsa_circ_0040571
Host gene    WWOX
Species    homo sapiens
Peptide    -
Sequence  TCACACCGAGGAGAAGACTCAGTGGGAACATCCAAAAACTGGAAAAAGAAAACGAGTGGCAGGAGATTTGCCATACGGATGGGAACAAGAAACTGATGAGAACGGACAAGTGTTTTTTGTTGACCATATAAATAAAAGAACCACCTACTTGGACCCAAGACTGGCGTTTACTGTGGATGATAATCCGACCAAGCCAACCACCCGGCAAAGATACGACGGCAGCACCACTGCCATGGAAATTCTCCAGGGCCGGGATTTCACTGGCAAAGTGGTTGTGGTCACTGGAGCTAATTCAGGAATAGGGTTCGAAACCGCCAAGTCTTTTGCCCTCCATGGTGCACATGTGATCTTGGCCTGCAGGAACATGGCAAGGGCGAGTGAAGCAGTGTCACGCATTTTAGAAGAATGGCATAAAGCCAAGGTAGAAGCAATGACCCTGGACCTCGCTCTGCTCCGTAGCGTGCAGCATTTTGCTGAAGCATTCAAGGCCAAGAATGTGCCTCTTCATGTGCTTGTGTGCAACGCAGCAACTTTTGCTCTACCCTGGAGTCTCACCAAAGATGGCCTGGAGACCACCTTTCAAGTGAATCATCTGGGGCACTTCTACCTTGTCCAGCTCCTCCAGGATGTTTTGTGCCGCTCAGCTCCTGCCCGTGTCATTGTGGTCTCCTCAGAGTCCCATCGATTTACAGATATTAACGACTCCTTGGGAAAACTGGACTTCAGTCGCCTCTCTCCAACAAAAAACGACTATTGGGCGATGCTGGCTTATAACAGGTCCAAGCTCTGCAACATCCTCTTCTCCAACGAGCTGCACCGTCGCCTCTCCCCACGCGGGGTCACGTCGAACGCAGTGCATCCTGGAAATATGATGTACTCCAACATTCATCGCAGCTGGTGGGTGTACACACTGCTGTTTACCTTGGCGAGGCCTTTCACCAAGTCCATG
Expression
Description  Down regulated transcript levels were detected for KIRKOS-71 particularly 24 hr post low (0.63 +/- 0.05 fold, p = 0.002)and medium (0.64 +/- 0.08 fold, p = 0.05) irradiation in SHEP. In contrast, KIRKOS-71 transcript levels were highly significantly increased in U2OS 24 hr post 0.25 Gy (1.52 +/- 0.09 fold increase, p = 0.0001) in comparison to sham irradiated control cells. Following medium dose exposure, this circRNA was similarly elevated post 4 hr and 24 hr (2.0 +/- 0.26 fold increase, p = 0.017; 1.3 +/- 0.1 fold increase, p = 0.006 respectively) compared to controls. Exosomes were extracted from SHEP and U2OS 24 hr following 2.5 Gy, 0.25 Gy or sham irradiation. KIRKOS-71 was significantly down regulated at this time point post 2.5 Gy in exosomes isolated from SHEP in comparison to sham irradiated controls (p = 0.002 and p = 0.000057, respectively). KIRKOS-71 was also significantly down regulated 24 hr post 0.25 Gy in exosomes from this cell line in comparison to sham.
Method    microarray, qRT-PCR
Sample    Human umbilical vein endothelial cells, neuroblastoma cell line SHEP, osteosarcoma cell line U2OS,
Function
Description  -
Method    -
in vitro    -
in vivo    -
Interaction
Target    QKI-5
Type    circRNA-protein
Sample    U2OS
Experiment    RNA pull-down, qPCR
Description    QPCR amplification confirmed the presence of the KIRKOS-71 associating with QKI-5 in U2OS.
ceRNA target    -
Reference
Pubmed ID    29108237
Trait/Disease    -
Title    The circRNA interactome-innovative hallmarks of the intra- and extracellular radiation response.
Authors    OLeary VB, Smida J, Matjanovski M, Brockhaus C, Winkler K, Moertl S, Ovsepian SV, Atkinson MJ.
Journal    Oncotarget. 2017