RefCirc
a reference database for circRNAs validated by experiments

circ-Amotl1 Details
Basic Information
circRNA    circ-Amotl1
Alias    -
circBase ID    hsa_circ_0004214
Host gene    AMOTL1
Species    homo sapiens
Peptide    -
Sequence  TTGAAGATCCTCTTTGTAACTTCCACTCCCCAAACTTCCTGAGGATCTCAGAGGTGGAAATGAGAGGTTCCGAGGATGCGGCAGCTGGAACAGTATTGCAGCGGCTGATCCAGGAACAACTGCGGTATGGCACCCCAACCGAGAACATGAACTTGCTGGCCATTCAGCACCAGGCCACAGGGAGTGCAGGACCAGCCCATCCTACAAACAACTTTTCTTCCACGGAAAACCTCACTCAAGAAGACCCACAAATGGTCTACCAGTCAGCACGCCAAGAACCGCAGGGTCAAGAACACCAGGTGGACAATACGGTGATGGAGAAACAGGTCCGGTCCACGCAGCCTCAGCAGAACAACGAGGAACTGCCCACTTACGAGGAGGCCAAAGCACAGTCGCAGTTCTTCAGGGGGCAGCAGCAGCAGCAACAGCAGCAGGGGGCGGTGGGCCATGGTTACTACATGGCAGGGGGCACCAGTCAGAAGTCCCGAACTGAGGGGAGGCCCACTGTGAACCGTGCCAACAGTGGACAGGCGCATAAGGACGAGGCGCTGAAGGAACTGAAGCAGGGCCACGTCCGCTCGCTCAGCGAGAGAATCATGCAGCTGTCCCTGGAGAGGAATGGGGCCAAGCAACACCTTCCCGGCTCGGGGAATGGAAAGGGCTTCAAAGTAGGAGGGGGGCCCTCCCCTGCCCAGCCTGCAGGTAAAGTGCTGGACCCTCGGGGTCCTCCACCTGAGTACCCCTTCAAGACCAAGCAAATGATGTCCCCAGTCAGCAAGACCCAGGAGCACGGACTTTTTTATGGTGACCAGCACCCCGGGATGCTCCACGAGATGGTCAAGCCCTACCCTGCTCCTCAGCCTGTGAGAACAGATGTGGCCGTCCTGCGGTACCAGCCACCCCCTGAGTATGGGGTAACGAG
Expression
Description  circ-Amotl1 was preferentially expressed in neonate cardiac tissue. neonatal cardiac tissues expressed higher levels of circ-Amotl1 relative to those from post-natal patients.
Method    qRT-PCR
Sample    neonate cardiac tissue, post-natal patients
Function
Description  circ-Amotl1 potentiates AKT-enhanced cardiomyocyte survival. In primary cardiomyocytes, epithelial cells, and endothelial cells, we found that forced circ-Amotl1 expression increased the nuclear fraction of pAKT. We further detected increased nuclear pAKT in circ-Amotl1-treated hearts. In vivo, circ-Amotl1 expression was also found to be protective against Doxorubicin (Dox)-induced cardiomyopathy.
Method    loss of function, gain of function
in vitro    primary cardiomyocytes, epithelial cells, endothelial cells, MCF cells, YPEN cells
in vivo    a mouse model of Dox-induced cardiomyopathy
Interaction
Target    AKT and PDK1
Type    circRNA-protein
Sample    MCF cells, YPEN cells
Experiment    RNA pull-down
Description    circ-Amotl1 physically binds to both PDK1 and AKT1, facilitating the cardio-protective nuclear translocation of pAKT.
ceRNA target    -
Reference
Pubmed ID    29109781
Trait/Disease    Cardiovascular Disease
Title    A Circular RNA Binds To and Activates AKT Phosphorylation and Nuclear Localization Reducing Apoptosis and Enhancing Cardiac Repair.
Authors    Zeng Y, Du WW, Wu Y, Yang Z, Awan FM, Li X, Yang W, Zhang C, Yang Q, Yee A, Chen Y, Yang F, Sun H, Huang R, Yee AJ, Li RK, Wu Z, Backx PH, Yang BB.
Journal    Theranostics. 2017