RefCirc
a reference database for circRNAs validated by experiments

cir-ITCH Details
Basic Information
circRNA    cir-ITCH
Alias    -
circBase ID    -
Host gene    ITCH
Species    homo sapiens
Peptide    -
Sequence  -
Expression
Description  low in ESCC
Method    RT-PCR, qRT-PCR
Sample    ESCC and corresponding paracancerous tissue samples, Eca-109, TE-1
Function
Description  As sponge of miR-7, miR-17, and miR-214, cir-ITCH might increase the level of ITCH. ITCH hyper expression promotes ubiquitination and degradation of phosphorylated Dvl2, thereby inhibiting the Wnt/beta-catenin pathway.
Method    gain of function
in vitro    Eca-109, TE-1
in vivo    4-5 weeks old female BALB/c nude mice
Interaction
Target    miR-7, miR-17, and miR-214
Type    circRNA-miRNA
Sample    Eca-109, TE-1
Experiment    luciferase reporter assay
Description    The results of the empty vector construct and miR-17 showed that luciferase activity was significantly reduced in a concentration-dependent manner in Eca-109 cells, and this trend was also present with mimics of miR-7, miR-214, miR-216b, and miR-128 in the control cells. However, in cells with cir-ITCH hyperexpression, the results showed that there were no significant differences in luciferase activity when the psiCHECK-2-ITCH-binding-site vector and miRNAs were co-transfected into Eca-109 cells except the miR-216b and miR-128.
ceRNA target    ITCH
Reference
Pubmed ID    25749389
Trait/Disease    Esophageal Squamous Cell Carcinoma
Title    Circular RNA ITCH has inhibitory effect on ESCC by suppressing the Wnt/beta-catenin pathway.
Authors    Li F, Zhang L, Li W, Deng J, Zheng J, An M, Lu J, Zhou Y.
Journal    Oncotarget. 2015