RefCirc
a reference database for circRNAs validated by experiments

circ-NT5C2 Details
Basic Information
circRNA    circ-NT5C2
Alias    -
circBase ID    -
Host gene    NT5C2
Species    homo sapiens
Peptide    -
Sequence  -
Expression
Description  Circ-NT5C2 was up-regulated in osteosarcoma
Method    microarray, qRT-PCR
Sample    osteosarcoma tissue and adjacent normal tissue, hFOB, MG-63, U2OS, SOSP-9607 and SAOS-2
Function
Description  circ-NT5C2 had an AUC (area under the curve) value of 0.753. Circ-NT5C2 silencing suppressed osteosarcoma cells proliferation in vitro. Circ-NT5C2 silencing suppressed osteosarcoma cells progression in vivo and in vitro.
Method    loss of function
in vitro    MG-63, U2OS
in vivo    male BALB/c nude mice (5-6 weeks)
Interaction
Target    miR-448
Type    circRNA-miRNA
Sample    HEK-293T
Experiment    luciferase reporter assay
Description    Luciferase reporter assay indicated that luciferase vitality was decreased when co-transfected with miR-448 mimics and circ-NT5C2 wild type, while miR-150 and miR-93 did not have the difference.
ceRNA target    -
Reference
Pubmed ID    29383123
Trait/Disease    Osteosarcoma
Title    Circular RNA circ-NT5C2 acts as an oncogene in osteosarcoma proliferation and metastasis through targeting miR-448.
Authors    Liu X, Zhong Y, Li J, Shan A.
Journal    Oncotarget. 2017