RefCirc
a reference database for circRNAs validated by experiments

circAFF1 Details
Basic Information
circRNA    circAFF1
Alias    -
circBase ID    -
Host gene    AFF1
Species    homo sapiens
Peptide    -
Sequence  -
Expression
Description  We indeed detected circAFF1 in the insulin-secreting cell lines INS832/13, and MIN6B1, as well as, in mouse, rat, and human islets.
Method    RNA-seq, microarray, qRT-PCR
Sample    INS832/13, MIN6B1, mouse islets, ratislets, human islets.
Function
Description  Silencing of circAFF1 in MIN6B1 cells or primary rat beta-cells resulted in an increase in apoptosis comparable to that observed after treatment of the cells with a combination of proinflammatory cytokines. In contrast, circAFF1 silencing did not affect the proliferative capacity of MIN6B1 cells, and did not alter insulin content or secretion in response to glucose of MIN6B1 cells or of primary rat b-cells.
Method    loss of function
in vitro    MIN6B1 cells
in vivo    primary rat beta-cells
Interaction
Target    -
Type    -
Sample    -
Experiment    -
Description    -
ceRNA target    -
Reference
Pubmed ID    29396373
Trait/Disease    Diabetes Mellitus
Title    Circular RNAs as novel regulators of beta-cell functions in normal and disease conditions.
Authors    Stoll L, Sobel J, Rodriguez-Trejo A, Guay C, Lee K, Veno MT, Kjems J, Laybutt DR, Regazzi R.
Journal    Mol Metab. 2018