RefCirc
a reference database for circRNAs validated by experiments

hsa_circ_0000977 Details
Basic Information
circRNA    hsa_circ_0000977
Alias    hsa_circ_001918
circBase ID    hsa_circ_0000977
Host gene    NOL10
Species    homo sapiens
Peptide    -
Sequence  TTCTGAAGTTTATAGGTTAAACTTAGAACAAGGACGATACCTGAATCCTCTACAAACTGATGCTGCGGAGAATAATGTTTGTGACATAAATTCAGTGCATGGCTTGTTTGCCACAGGAACCATAGAGGGTAGAGTGGAGTGCTGGGACCCAAGAACTCGAAACAGAGTTGGCCTGTTAGACTGCGCCTTAAACAGTGTCACAGCAGATTCAGAGATAAACAGTTTACCAACAATCTCTGCTTTGAAATTTAATGGTGCCTTGACCATGGCAGTTGGAACAACCACAGGGCAGGTTTTATTATATGACCTTCGATCTGATAAGCCATTGCTAGTTAAAGATCACCAGTATGGGCTGCCCATTAAGTCCGTTCATTTCCAGGATTCATTAGATCTGATTTTGTCTGCTGACTCTCGAATTGTCAAGATGTGGAATAAGAACTCCGGAAAAATATTTACTTCCTTGGAGCCAGAGCATGACCTTAATGATGTTTGTCTCTACCCCAACTCAGGCATGCTTCTGACGGCCAATGAAACCCCCAAGATGGGCATCTATTACATTCCA
Expression
Description  upregulated
Method    microarray, qRT-PCR
Sample    AsPC-1 and PANC-1, matched PDAC and adjacent non-cancerous tissues
Function
Description  Overexpression of hsa_circ_0000977 is correlated with poor prognosis in PDAC patients. hsa_circ_0000977 regulates cell proliferation and cell cycle by sequestering miR-874-3P in vitro. Knockdown of hsa_circ_0000977 inhibits pancreatic cell growth in vivo.
Method    gain of function
in vitro    AsPC-1 and PANC-1
in vivo    Five-week-old female BALB/c nude mice
Interaction
Target    miR-874-3p
Type    circRNA-miRNA
Sample    HEK293T, AsPC-1 and PANC-1
Experiment    luciferase reporter assay, RNA FISH
Description    There was significant reduction of firefly luciferase reporter activity for transfected hsa-miR-874-3p and hsa-circ-0000977 WT, and there was no notable change of luciferase reporter activity in the hsa-circ-0000977 mutant. The co-localization of hsa-circ-0000977 and miR-874-3p in pancreatic cancer cells by FISH.
ceRNA target    PLK1
Reference
Pubmed ID    29454093
Trait/Disease    Pancreatic Ductal Adenocarcinoma
Title    Silencing circular RNA hsa_circ_0000977 suppresses pancreatic ductal adenocarcinoma progression by stimulating miR-874-3p and inhibiting PLK1 expression.
Authors    Huang WJ, Wang Y, Liu S, Yang J, Guo SX, Wang L, Wang H, Fan YF.
Journal    Cancer Lett. 2018