RefCirc
a reference database for circRNAs validated by experiments

circ-GRB10 Details
Basic Information
circRNA    circ-GRB10
Alias    -
circBase ID    hsa_circ_0080210
Host gene    GRB10
Species    homo sapiens
Peptide    -
Sequence  ATATTCTGGAGGAAGGTGTGAACGCAACTTTGCCTCCCAGGGAACAAACATCCTCCTTCTAAGTGGTAGATGTGGGTGAGCTGACCCTGCTGGAGTCTGTCCCCTGGGCTACCCTCTGCTTCCCCCCATTGTGAGTGGTCCGTGAAGCACAGCGTTGACCAGACCTAAACCTGTTTGCTCCCAGGACAAGGTGGAGCAGACACCTCGCAGTCAACAAGACCCGGCAGGACCAGGACTCCCCGCACAGTCTGACCGACTTGCGAATCACCAGGAGGATGATGTGGACCTGGAAGCCCTGGTGAACGATATGAATGCATCCCTGGAGAGCCTGTACTCGGCCTGCAGCATGCAGTCAGACACGGTGCCCCTCCTGCAGAATGGCCAGCATGCCCGCAGCCAGCCTCGGGCTTCAGGCCCTCCTCGGTCCATCCAGCCACAGGTGTCCCCGAGGCAGAGGGTGCAGCGCTCCCAGCCTGTGCACATCCTCGCTGTCAGGCGCCTTCAGGAGGAAGACCAGCAGTTTAGAACCTCATCTCTGCCGGCCATCCCCAATCCTTTTCCTGAACTCTGTGGCCCTGGGAGCCCCCCTGTGCTCACGCCGGGTTCTTTACCTCCGAGCCAGGCCGCCGCAAAGCAG
Expression
Description  circ-GRB10 was significantly downregulated in intervertebral disc degeneration (IDD) nucleus pulposus (NP) tissues compared with normal NP tissues.
Method    microarray, qRT-PCR
Sample    intervertebral disc degeneration (IDD) nucleus pulposus (NP) tissues, normal NP tissues
Function
Description  Overexpression of circ-GRB10 inhibited NP cell apoptosis. circ-GRB10 could sequester miR-328-5p, which could potentially lead to the upregulation of target genes related to cell proliferation via the ErbB pathway. circ-GRB10/miR-328-5p/ERBB2 signaling pathway is involved in IDD development, suggesting that circ-GRB10 might be a novel therapeutic target for IDD.
Method    loss of function, gain of function
in vitro    nucleus pulposus cells
in vivo    -
Interaction
Target    miR-328-5p
Type    circRNA-miRNA
Sample    nucleus pulposus cells
Experiment    FISH, luciferase reporter assay
Description    These data suggest that miR-328-5p directly targets circ-GRB10 in vitro.
ceRNA target    ERBB2
Reference
Pubmed ID    29476072
Trait/Disease    Intervertebral Disc Degeneration
Title    Circular RNA GRB10 as a competitive endogenous RNA regulating nucleus pulposus cells death in degenerative intervertebral disk.
Authors    Guo W, Zhang B, Mu K, Feng SQ, Dong ZY, Ning GZ, Li HR, Liu S, Zhao L, Li Y, Yu BB, Duan HQ, Sun C, Li YJ.
Journal    Cell Death Dis. 2018